The TORCH Rapid Test Device (Whole Blood/Serum/Plasma) is a panel of rapid qualitative lateral flow test designed for the qualitative detection of IgM/IgG antibodies to Toxoplasma gondii(TOXO),Cytomegalovirus (CMV), Rubella, Herpes Simplex Virus (HSV-1 and HSV-2) in human serum/plasma samples.
Principle of TORCH Combo Rapid Test Device
The TORCH IgM/IgG rapid test is an immuno-chromatographic assay, based on the principle of Gold Immuno-chromatography Assay (GICA) principle. Recombinant antigens of the five viruses and anti-human IgM antibodies to the five viruses are respectively used to detect the specific antibodies in the human serum or plasma samples.
When the samples are added to the sample wells on the test panel, the antibody, say TOXO IgM antibody, will react with the anti-human TOXO IgM antibody in the membrane strip, and complexes will emerge. These complexes move along the membrane strip chromatographically to the test region (T), where these complexes will be captured by the pre-coated recombinant TOXO antigen. Then a red or pink line will appear, indicating a positive result. The unbounded complex moves on to the control region (C), where they are captured by the anti-mouse antibody, and a red line will appear, indicating the assay is a valid one. So the control line provides an inner quality control mechanism.
The test principle of all the other four antibodies is the same as that of the TOXO IgM testing. The color intensity is in accordance with the concentration of the respective antibody to the related TORCH virus in the samples.