The h-FABP Test Device is intended for the qualitative determination of cardiac FABP in human whole blood. Measurement of FABP values is useful in the evaluation of acute myocardial infarction (AMI).
Knowledge of FABP Test Device
The human cardiac FABP (H-FABP or FABP3) is a 132 amino acid small molecular weight (15kDa) protein. H-FABP is one of the most abundant proteins in cardiomyocytes and is mainly involved in fatty acid transport. The concentration of fatty acids in plasma and in myocardial tissue rise during myocardial ischemia acting to protect myocytes against fatty acid oxidation. Upon acute myocardial infarction (AMI) the blood and urine levels of H-FABP rise rapidly (within 1-3 hours) reaching their peak values at 6 hours, and return to baseline concentration within 24 hours. Characteristics of H-FABP appear to be similar to those of myoglobin, however, H-FABP concentrations are 2–10-fold higher in the heart than in the skeletal muscle. In contrast, myoglobin concentrations are 2-fold lower in cardiac cells than skeletal cells. Thus, the apparent greater cardiospecificity of H-FABP, along with certain aspects of its biology account for its greater value in diagnostics of AMI.